LOCUS       sequence-148111-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genetically encoded pH sensor made from mutagenesis of the red 
            fluorescent protein mKeima.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Tantama M, Hung YP, Yellen G
  TITLE     Imaging Intracellular pH in Live Cells with a Genetically Encoded 
            Red Fluorescent Protein Sensor.
  JOURNAL   J Am Chem Soc. 2011 Jul 6. 133(26):10034-7.
  PUBMED    21631110
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2011 Jul 6. 133(26):10034-7."
COMMENT     Sequence Label: GW1-pHRed
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(171..830,831..833,834..836))
                     /codon_start=1
                     /product="red fluorescent protein that serves as a 
                     ratiometric pH sensor (Tantama et al., 2011)"
                     /label=pHRed
                     /note="contains an A213S mutation relative to the parental 
                     mKeima protein"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEISIARHSLLG
                     "
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc aatgaattcg
      121 tcgactcagc tagcaccacc agagccacca ccgtcggcgg agttgcctgt ccccagcaga
      181 gagtggcggg cgatagagat ttcacactgc tcaacgctgg tgtagtcgcg gttgtgagag
      241 gtaacatcca gcttgcggtc gatttcgtgg cggccaggca tgcgaacagg ctttttagcc
      301 ttgtaagtgc tcttgaactc gcacaggtag tgaccgccgc cttccagttt cagggccata
      361 taatcgttac caatcagcat accgtcgcgg gcaaacaggc gttcggtgct gggctcccag
      421 ccttgggttt tcttctgcat aactgggccg ttaggtggga agttctcgcc ggaaatctta
      481 acgttgtaga tgaagcagtt accctggatg gagctgtcgt tggaaacggt acaaactgcg
      541 ccgtcctcga agttcataga gcgctcccag gtgtagccct ctgggaagct ctgcttaaag
      601 tagtcaggga tgtcctcggg gtatttggtg aaagggatgg agccgtattg gagctgaggg
      661 gacaggatat cccaggcgaa aggcagtggg ccgcctttgg taacggtcag cttaacggtc
      721 tgctcgccct cgtaaggctt gcccttgccg tcaccttcaa cctcgaagta gtggccgtta
      781 acggtgccag acatataaac tttataggtc atttgctttg cgataacgga aaccatggtg
      841 gtcta
//
