LOCUS       sequence-148128-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE TRE dCas9-KRAB
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..42
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    66..84
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    150..168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        285..323
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     320..344
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    453..477
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
       61 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      121 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
      181 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
      241 cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt
      301 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
      361 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaac
      421 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctggc
      481 gccaccatgg acaagaagta ctccattggg ctcgctatcg gtaccaacag cgtcggctgg
      541 gccgtcatta cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc
      601 gatcgccaca gcataaagaa gaacctcatt ggagccctcc tgttcgactc cggggagacg
      661 gccgaagcca cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg
      721 atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc
      781 cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc
      841 tttggcaata tcgtggacga ggtggcgtac catg
//
