LOCUS       sequence-148131-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ISce1 restriction site is flanked by Tet operator repeats, 96 on 
            each side.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T
  TITLE     Spatial dynamics of chromosome translocations in living cells.
  JOURNAL   Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
  PUBMED    23929981
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-08-9- 9"; volume: "341"; issue: 
            "6146"; pages: "660-4"
COMMENT     Sequence Label: pTetO-ISce1-TetO
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        77..115
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     112..136
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(225..245)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(225..243)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(277..299)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    288..304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(312..318,319..336,337..342))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    357..378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(495..512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
       61 tcgtcgaccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
      121 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      181 ccgggaccga tccagcctcc gcggtggagc tccagctttt gttcccttta gtgagggtta
      241 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      301 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      361 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      421 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      481 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      541 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      601 aagaacatgt gagcaaaagg ccagcaaaag gccagga
//
