LOCUS       sequence-148133-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ISce1 restriction site is flanked by Tet operator repeats, 96 on 
            each side.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D, Misteli T
  TITLE     Spatial dynamics of chromosome translocations in living cells.
  JOURNAL   Science. 2013 Aug 9;341(6146):660-4. doi: 10.1126/science.1237150.
  PUBMED    23929981
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-08-9- 9"; volume: "341"; issue: 
            "6146"; pages: "660-4"
COMMENT     Sequence Label: pTetO-ISce1-TetO
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..28
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10..28
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(117..141)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(138..176)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    203..221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    245..263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(300..319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(301..319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(329..346)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(329..345)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(338..360)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(624..645)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     837..856
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggaggctg
       61 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
      121 tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg cctacctcga
      181 cccggtcgac gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
      241 cttttctcta tcactgatag ggagtggtaa actcgagggg gggcccggta cccaattcgc
      301 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
      361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      421 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      481 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      541 ctcatttttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      601 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      661 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      721 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      781 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaaggaaggg
      841 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
//
