LOCUS       sequence-148137-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral packaging plasmid; Contains Rev; also 
            requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid 
            (Addgene#12259).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L
  TITLE     A third-generation lentivirus vector with a conditional packaging 
            system.
  JOURNAL   J Virol. 1998 Nov . 72(11):8463-71.
  PUBMED    9765382
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1998 Nov . 72(11):8463-71."
COMMENT     Sequence Label: pRSV-Rev
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    350..371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(386..391,392..409,410..416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     429..451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     448..464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     448..464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        609..870
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     881..905
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      361 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
      481 tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc gaaaagcggg
      541 gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct tttgcatagg
      601 gagggggaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
      661 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      721 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      781 actgaattcc gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacgcc
      841 atttgaccat tcaccacatt ggtgtgcacc tccaagctcg agctcgttta gtgaaccgtc
      901 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      961 ccagcctccc ctcgaagcta gtcgattag
//
