LOCUS       sequence-148153-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Full length RCAN1 expression with CMV promoter.  Flag tagged..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Liu Q, Busby JC, Molkentin JD
  TITLE     Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a 
            signalling nodal control point.
  JOURNAL   Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 
            2009 Jan 11.
  PUBMED    19136967
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb1823"; 
            journalName: "Nat Cell Biol"; date: "2009-02"; volume: "11"; issue: 
            "2"; pages: "154-61"
COMMENT     Sequence Label: RCAN1
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      704..713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    717..851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(754..773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     808..827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(943..963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat cgatatggac
       61 tacaaagacg atgacgacaa gcgaattccc cggggatcca tggaggaggt ggatctgcag
      121 gacctgccga gcgccaccat cgcctgccac ctggacccgc gcgtgttcgt ggacggcctg
      181 tgccgggcca aatttgaatc cctcttcaga acatatgaca aggacaccac cttccagtat
      241 tttaagagct tcaaacgtgt ccggataaac ttcagcaacc ccttatctgc agccgatgcc
      301 aggctgcggc tgcacaagac cgagttcctg gggaaggaaa tgaagttgta ttttgctcag
      361 actttacaca taggaagttc acacctggct ccgcccaatc ccgacaaaca gttcctcatc
      421 tcccctccgg cctctcctcc cgttggctgg aaacaagtag aagatgccac ccccgtcata
      481 aattacgatc ttttatatgc catctccaag ctggggccag gagagaagta tgaactgcat
      541 gcagcgacag acaccactcc cagtgtggtg gtccacgtgt gtgagagtga ccaagagaat
      601 gaggaggaag aggaagagat ggagagaatg aagagaccca agcccaaaat catccagaca
      661 cggagaccgg agtacacacc gatccacctt agctgaaagc ttggccgcca tggcccaact
      721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      841 atgtctggat cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaaagag
      901 gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
      961 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     1021 agtcagcaac caggtgtgga aagtcccc
//
