LOCUS       sequence-148159-        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photinus pyralis sulfotransferase ST2 - pHis8-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Fallon TR, Li FS, Vicent MA, Weng JK
  TITLE     Sulfoluciferin is biosynthesized by a specialized luciferin 
            sulfotransferase in fireflies.
  JOURNAL   Biochemistry. 2016 May 26.
  PUBMED    27227579
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2016 May 26."
COMMENT     Sequence Label: pJKW 0633
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     386..405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             479..502
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             509..529
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      421 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgaaaca
      481 ccaccaccac caccaccacc acggtggtga aaacttgtac ttccaggccc atggcatgga
      541 agaaaataac tatctccctt atttgtgtga aaaaaccgca aggagtcttt gcgccaaaat
      601 caaaccgttt gaagttaaaa atgacgacat ttttgtaatt ggagcgacaa gatcgggtac
      661 catgtgggtg caggaaatgg tttggttgat tgcaaatgat ttggattatg agaaagctaa
      721 agagggggcg tactattggg ttccacttct ggaatatagt gtttatttgt gcagcaaacc
      781 gggctctcag tcttatcata tgcaagagcg gtacgagcct aattcaatag agttttgtaa
      841 aaacttaaaa agtccacgca taattaaaac gcatttacca gaagaatatc taccacggca
      901 actgtttgat aaagaaaagg aaactaagat tatttacatt gcacgcaacc ccaaggatgt
      961 ctgcttgtca ggatatcatt ttaaaagaat tgttatgaag tgcgaatttg aatccctgga
     1021 gagctatgcc gaagttttca tgtcggattc atttcccaaa ggagattatt ggaagaatgt
     1081 gctgtacttt t
//
