LOCUS       sequence-148164-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His tagged GINA3 protein in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, 
            Kufareva I, Abagyan R, Ghosh P
  TITLE     Structural basis for activation of trimeric Gi proteins by multiple 
            growth factor receptors via GIV/Girdin.
  JOURNAL   Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 
            10.1091/mbc.E14-05-0978. Epub 2014 Sep 3.
  PUBMED    25187647
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-05-0978"; journalName: "Mol Biol Cell"; date: 
            "2014-11-5- 5"; volume: "25"; issue: "22"; pages: "3654-71"
COMMENT     Sequence Label: His-GINA3
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatggg ctgcacgttg agcgccgagg
      241 acaaggcggc ggtggagcgg agtaagatga tcgaccgcaa cttgcgggag gacggagaga
      301 aagcggccaa agaagtgaag ctgctgctgc tcggcgctgg agaatctggt aaaagtacta
      361 ttgtgaaaca gatgaaaatc attcatgagg atggctattc cgaggacgag tgtaagcagt
      421 ataaagttgt cgtctacagc aataccattc agtccatcat tgcaatcata agagccatgg
      481 gacggttgaa gattgatttt ggggaagctg ccagagcgga tgatgcccga cagttatttg
      541 ttttagctgg cagtgctgag gaaggagtca tgacttcaga actagcaggc gtgattaaac
      601 gtttatggcg agatggcggg gtgcaggcat gcttcagcag gtccagggaa tatcagctca
      661 atgattctgc ttcatattac ctaaatgatt tggatagaat atcccagacc aactacattc
      721 caactcagca agatgttctt cggacgagag tgaagactac aggcattgtg gagacccact
      781 tcaccttcaa ggagctctac ttcaaaatgt ttgatgtagg tggccaaaga tccgaacgga
      841 aaaagtggat ccactgtttt gagggagtga cagcaattat cttttgtgtg gctctcagtg
      901 attacgacct tgttctggct gaggacgagg aaatgaaccg aatgcatgaa agcatgaaat
      961 tgtttgacag catttgtaac aacaaatg
//
