LOCUS       sequence-148166-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PICK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Anggono V, Clem RL, Huganir RL
  TITLE     PICK1 loss of function occludes homeostatic synaptic scaling.
  JOURNAL   J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 
            10.1523/JNEUROSCI.5633-10.2011.
  PUBMED    21307255
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5633-10"; journalName: "J Neurosci"; date: 
            "2011-02-9- 9"; volume: "31"; issue: "6"; pages: "2188-96"
COMMENT     Sequence Label: pSuper-PICK1 shRNA
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(143..161)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(182..198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(182..198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(195..217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    206..222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(230..236,237..254,255..260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    275..296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(413..430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(664..683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgtctttg gatttgggaa tcttataagt tctgtatgag accacagatc cccgcctcac
       61 catcaagaag taccttcaag agaggtactt cttgatggtg aggctttttt ctcgaggggg
      121 ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
      181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      241 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      301 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      361 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      421 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      481 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      541 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      601 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      661 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      721 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      781 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      841 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      901 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      961 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1021 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaa
//
