LOCUS       sequence-148167-        1139 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PICK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Anggono V, Clem RL, Huganir RL
  TITLE     PICK1 loss of function occludes homeostatic synaptic scaling.
  JOURNAL   J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 
            10.1523/JNEUROSCI.5633-10.2011.
  PUBMED    21307255
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5633-10"; journalName: "J Neurosci"; date: 
            "2011-02-9- 9"; volume: "31"; issue: "6"; pages: "2188-96"
COMMENT     Sequence Label: pSuper-PICK1 shRNA
FEATURES             Location/Qualifiers
     source          1..1139
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(82..296)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(142..161)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(316..332)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(316..332)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(364..383)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(365..383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(390..407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(390..406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(399..421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      548..1003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(635..654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     845..866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1029..1133
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgggcccccc ctcgagaaaa aagcctcacc atcaagaagt acctctcttg aaggtacttc
       61 ttgatggtga ggcggggatc tgtggtctca tacagaactt ataagattcc caaatccaaa
      121 gacatttcac gtttatggtg atttcccaga acacatagcg acatgcaaat attgcagggc
      181 gccactcccc tgtccctcac agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc
      241 cgcctagtga cactgggccc gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat
      301 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccaat
      361 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      601 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      661 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      721 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      781 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      841 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      901 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      961 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     1021 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1081 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagt
//
