LOCUS       sequence-148171-        6557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myc-PICK1 shRNA resistant cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Anggono V, Clem RL, Huganir RL
  TITLE     PICK1 loss of function occludes homeostatic synaptic scaling.
  JOURNAL   J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 
            10.1523/JNEUROSCI.5633-10.2011.
  PUBMED    21307255
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.5633-10"; journalName: "J Neurosci"; date: 
            "2011-02-9- 9"; volume: "31"; issue: "6"; pages: "2188-96"
COMMENT     Sequence Label: pRK5-myc-PICK1-rescue
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      963..972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             972..1001
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      2802..2811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2815..2949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2852..2871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2906..2925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3022..3379
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3022..3213
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3045..3065)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3230..3365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3399..3416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3399..3415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3408..3430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3628..4083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3715..3734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3925..3946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4100..4119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4254..4276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4329..4347)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4415..4519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4520..4588,4589..5380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4738..4757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5551..6139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6040..6059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6293..6310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(6463..6468,6469..6486,6487..6493)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6501..6517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6506..6528
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6525..6541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6525..6541
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataac cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc
      961 gcgccaccat ggagcaaaag ctcatttctg aggaagatct caatggtggt ggtggtgggt
     1021 cgacctttgc agacttagac tatgacatcg aggaggataa attaggaatc cccactgtgc
     1081 ccgggaaggt caccctgcag aaggatgctc agaacctgat tggcatcagc atcggaggag
     1141 gggcccagta ctgtccttgt ctctacattg tccaggtatt tgataacacg cctgcagcct
     1201 tggacggcac tgtggcagct ggtgatgaga tcactggggt caatggccga tcaatcaaag
     1261 gaaaaaccaa ggtagaggtg gcgaagatga ttcaggaggt gaagggggag gtgaccatcc
     1321 attacaacaa gctgcaggcg gaccctaagc agggcatgtc cctggacatt gtgttgaaga
     1381 aagtcaagca ccggctggta gaaaacatga gttcaggcac agctgatgcc ctgggtctaa
     1441 gccgtgccat cctgtgtaat gatgggttgg taaagcggct ggaggagctg gagcgcactg
     1501 ccgagctgta caaaggaatg acggagcaca ccaagaatct cctacgggcc ttttatgagc
     1561 tgtcacagac tcaccgggct tttggggacg tgttctctgt gattggggtg cgggagccgc
     1621 agccagcagc aagtgaagca tttgtgaagt ttgctgacgc acaccgcagc attgagaagt
     1681 tcggcattcg gctactgaaa accatcaaac cgatgctgac ggatctgaac acgtacctca
     1741 ataaggccat ccctgataca cgcctcacga taaaaaaata tctggatgtg aagtttgagt
     1801 acttgtcata ctgtctgaag gtgaaggaga tggacgacga ggagtacagc tgcattgccc
     1861 taggagagcc gctataccga gtgagcacag gcaactatga gtaccgcctt atcctgcgct
     1921 gccgccagga ggctcgagcc cgcttctccc agatgcgcaa ggatgtgctg gagaagatgg
     1981 agctgctgga ccagaagcac gtccaggaca tcgtgttcca gctgcagcgc ttcgtgtcta
     2041 ccatgtccaa gtactataat gactgctatg cggtgctgcg ggacgccgac gtcttcccca
     2101 tcgaggtgga cctggcacac accactctag cctacggccc taaccagggc ggctttaccg
     2161 atggcgaaga tgaggaggag gaggaggagg acggggcagc tagggaagtg tccaaggacg
     2221 cacgtggggc cactgggcct acagacaagg gcggaagctg gtgtgactcc tgagtgcccc
     2281 cctcaagtgg acctgggggc agggtagtgg gaggaccgca gtgggagtgg ggccgggcca
     2341 ctgtaggaca gcgaaggcat atagtggcgc ataaaagcct gctggctcgg ggcgcctgcc
     2401 tccctgctcc tctgtccttg aacagtgaac ccagcctgca gcctgggacc tggactctag
     2461 cccccatcct tccttcccac ctcccagcta gagggagagc tgggccttgg acagaaagag
     2521 aaggggctgg agcaggcaga gcacaggacc tgtccctgtc cctgtgagca cctcggtggt
     2581 gccacgcctg cagggaatgg gagctgggtg gacatccgag gctgtgacca gtgtaccagg
     2641 gcggccccct gagtccatgt gcacacctcg gcagcctata tttatttgtt tctggcttag
     2701 cctcaagggc tggaagctgg gcctgtattg aataaacaga aacccagaca gaaaaaaaaa
     2761 aaaaaagggc ggccgctaag taagtaagga tccccagctt ggccgccatg gcccaacttg
     2821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2941 gtctggatcg atcgggaatt aattcggcgc agcaccatgg cctgaaataa cctctgaaag
     3001 aggaacttgg ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3061 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3121 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3181 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3301 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3361 aggcctaggc ttttgcaaaa agctgttaac agcttggcac tggccgtcgt tttacaacgt
     3421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccccttc
     3481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgtagc
     3541 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3601 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     3661 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3721 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3781 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3841 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3901 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3961 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4021 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     4081 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc caactccgct
     4141 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     4201 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4261 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagt attcttgaag
     4321 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4381 ttagacgtca ggtttcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4741 atgtggcgcg gtattatccc gtgatgacgc cgggcaagag caactcggtc gccgcataca
     4801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5041 cgagcgtgac accacgatgc cagcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5941 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6301 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6361 gattcattaa tccagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6421 cgcaattaat gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6481 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6541 ccatgattac gaattaa
//
