LOCUS       sequence-148174-        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PACSIN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, 
            Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL
  TITLE     PICK1 interacts with PACSIN to regulate AMPA receptor 
            internalization and cerebellar long-term depression.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 
            10.1073/pnas.1312467110. Epub 2013 Aug 5.
  PUBMED    23918399
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312467110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-20- 20"; volume: "110"; issue: "34"; pages: 
            "13976-81"
COMMENT     Sequence Label: pSuper-PACSIN1-shRNA
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(138..156)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(177..193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(177..193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(190..212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    201..217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(225..231,232..249,250..255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    270..291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(408..425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(659..678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgtctttg gatttgggaa tcttataagt tctgtatgag accacagatc cccgcgccag
       61 ctcatcgaga aattaagaga tttctcgatg agctggcgct tttttctcga gggggggccc
      121 ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
      181 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      241 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      301 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaa
//
