LOCUS       sequence-148175-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA against PACSIN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, 
            Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL
  TITLE     PICK1 interacts with PACSIN to regulate AMPA receptor 
            internalization and cerebellar long-term depression.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 
            10.1073/pnas.1312467110. Epub 2013 Aug 5.
  PUBMED    23918399
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312467110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-20- 20"; volume: "110"; issue: "34"; pages: 
            "13976-81"
COMMENT     Sequence Label: pSuper-PACSIN1-shRNA
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(64..278)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(124..143)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(298..314)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..314)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(346..365)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(347..365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(372..389)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(372..388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(381..403)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      530..910
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(617..636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     827..848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagaaaaaag cgccagctca tcgagaaatc tcttaatttc tcgatgagct ggcgcgggga
       61 tctgtggtct catacagaac ttataagatt cccaaatcca aagacatttc acgtttatgg
      121 tgatttccca gaacacatag cgacatgcaa atattgcagg gcgccactcc cctgtccctc
      181 acagccatct tcctgccagg gcgcacgcgc gctgggtgtt cccgcctagt gacactgggc
      241 ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga attcctgcag cccgggggat
      301 ccactagttc tagagcggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg
      361 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
      421 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
      481 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
      541 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      601 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      661 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      721 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      781 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
      841 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
      901 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaa
//
