LOCUS       sequence-148177-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, 
            Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL
  TITLE     PICK1 interacts with PACSIN to regulate AMPA receptor 
            internalization and cerebellar long-term depression.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 
            10.1073/pnas.1312467110. Epub 2013 Aug 5.
  PUBMED    23918399
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312467110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-20- 20"; volume: "110"; issue: "34"; pages: 
            "13976-81"
COMMENT     Sequence Label: pRK5-H1-shRNA-CMV-HA-PACSIN1
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      382..391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             394..420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgattgaa ttcccgaacc gacagtcggt
      361 ctcttcacca aggccattcg cgccaccatg ggctacccct atgatgtgcc tgactacgca
      421 gatctcaatg gtggtggtgg tgggtcgacc atgtctggct cctacgatga ggcctcagag
      481 gagatcacag atagcttctg ggaggtgggg aactacaagc ggacggtgaa gcgcatcgac
      541 gatgggcacc gcctgtgcaa cgacctcatg agctgcgtgc aggagcgcgc caagatcgag
      601 aaggcatacg cgcagcagct caccgactgg gccaagcgct ggcgccaact tattgaaaag
      661 ggtcctcagt atggcagcct ggagcgggcg tggggcgcca tgatgacaga agcagataag
      721 gtcagcgagc tgcaccagga ggtgaagaac agcctgctga atgaggacct ggagaaagtc
      781 aagaactggc agaaggatgc ctatcacaag cagatcatgg gtggcttcaa ggagacgaaa
      841 gaggccgagg atggcttccg aaaggcccag aagccctggg ctaaaaagat gaaggagcta
      901 gaggcggcca agaaggccta tcacttggct tgtaaggagg aaaggctggc catgacccgg
      961 gagatgaaca gtaagacaga gcagtcggtc acccctgaac agcagaagaa acttgtggac
     1021 aaagtggaca aatgcagaca ggatgtgcaa aagactcag
//
