LOCUS       sequence-148178-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, 
            Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL
  TITLE     PICK1 interacts with PACSIN to regulate AMPA receptor 
            internalization and cerebellar long-term depression.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 
            10.1073/pnas.1312467110. Epub 2013 Aug 5.
  PUBMED    23918399
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1312467110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-20- 20"; volume: "110"; issue: "34"; pages: 
            "13976-81"
COMMENT     Sequence Label: pRK5-H1-shRNA-CMV-HA-PACSIN1
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attataagct gcaataaaca agttgggcca tggcggccaa gctggggatc cttacttact
       61 tagcggccgc ctatatagcc tcaacgtagt tggcaggata gaggcccagc tgtccgctgt
      121 ccagccgccc gcggcaccaa ccctgttcgt cttcctctcc gagcttggtg agctcatctc
      181 cggccttgaa gctgagctca tcctgctcct gaccgtcgta gtcatagagt gcccgtacac
      241 gaactccctt ggcatcatcc tcgaaggggt tggcgccacc attggcctca ttgcccccga
      301 aggggtttcc gctctcatcg tctgaccact cggtggcata tgtttggcct cggtcatagc
      361 tgctaacact gccacggtcc ccagcctggg atgtggattc tacagcccca gtggcattgc
      421 tcagggtggc cccctctgcc ttcttaggct gtttctcctt cttggcagtg gtgtgcggga
      481 ggtctgggtt ccactcctcg aactgcggcc agttcatggg catcccgggg ccactggtgc
      541 tgcggaacca cctgaggtcc tcctgggcat cggccccccg gatggcctgc tccagttctc
      601 ggtagacatg catgtagctg ctgttctccg ctaggttgag atgccgtttg atatccagca
      661 ggacttcctt caggaagacc agccgcttct cctcaaactg ctggcactgc tcaaacacct
      721 gctccatgcc ctccatgtac tgtggtgtgg tcttgcccac atcttccagc accttctcat
      781 acttctcctg agtcttttgc acatcctgtc tgcatttgtc cactttgtcc acaagtttct
      841 tctgctgttc aggggtgacc gactgctctg tcttactgtt catctcccgg gtcatggcca
      901 gcctttcctc cttacaagcc aagtgatagg ccttcttggc cgcctctagc tccttcatct
      961 ttttagccca gggcttctgg gcctttcgga agccatcctc ggcctctttc gtctccttga
     1021 agccacccat gatctgcttg tgataggcat ccttctgcca gttcttgact ttctccaggt
     1081 cctcattcag caggctgttc ttcacctcct gg
//
