LOCUS       sequence-148184-       11222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HOXA9 after doxycycline administration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11222)
  AUTHORS   Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, 
            Bernstein ID, Collins JJ, Zon LI, Daley GQ
  TITLE     Induction of multipotential hematopoietic progenitors from human 
            pluripotent stem cells via respecification of lineage-restricted 
            precursors.
  JOURNAL   Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 
            10.1016/j.stem.2013.09.002.
  PUBMED    24094326
REFERENCE   2  (bases 1 to 11222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.09"; journalName: "Cell Stem Cell"; date: 
            "2013-10-3- 3"; volume: "13"; issue: "4"; pages: "459-70"
COMMENT     Sequence Label: pINDUCER21-HOXA9
FEATURES             Location/Qualifiers
     source          1..11222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(266..271,272..289,290..296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     328..344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     328..344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     394..413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             605..1238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1285..1410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1907..2140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2325..2369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2662..2779
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2834..2852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2876..2894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2960..2978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3002..3020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3053..3091
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3088..3112
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3221..3245
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3500..3517
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(4071..4095)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4148..4174
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4394..5572
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4625..5563
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5520..5540
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5642..5675
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5682..6299,6300..6305,6306..6341,6342..6344,6345..6380,
                     6381..6383,6384..6419,6420..6428)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6466..7038
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6632..6649)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6859..6878
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7054..7056,7057..7059,7060..7773)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7054..7056,7057..7059,7060..7770)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7099..7120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7360..7379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7707..7728
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7909..7928
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8216..8804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8269..8289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8687..8698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8879..9112
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9231..9249)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9317..9421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9422..9490,9491..10282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9640..9659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10453..11041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10942..10961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11195..11212
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
       61 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
      181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
      361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
      541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      601 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      661 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      721 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      781 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      841 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      901 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      961 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     1021 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     1081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1741 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2641 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2701 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2761 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2821 gatcctttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2881 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2941 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3001 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     3061 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     3121 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     3181 ccccgaacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     3241 aggctccacc atggccacca ctggggccct gggcaactac tacgtggact cgttcctgct
     3301 gggcgccgac gccgcggatg agctgagcgt tggccgctat gcgccgggga ccctgggcca
     3361 gcctccccgg caggcggcga cgctggccga gcaccccgac ttcagcccgt gcagcttcca
     3421 gtccaaggcg acggtgtttg gcgcctcgtg gaacccagtg cacgcggcgg gcgccaacgc
     3481 tgtacccgct gcggtgtacc accaccatca ccaccacccc tacgtgcacc cccaggcgcc
     3541 cgtggcggcg gcggcgccgg acggcaggta catgcgctcc tggctggagc ccacgcccgg
     3601 tgcgctctcc ttcgcgggct tgccctccag ccggccttat ggcattaaac ctgaaccgct
     3661 gtcggccaga aggggtgact gtcccacgct tgacactcac actttgtccc tgactgacta
     3721 tgcttgtggt tctcctccag ttgatagaga aaaacaaccc agcgaaggcg ccttctctga
     3781 aaacaatgct gagaatgaga gcggcggaga caagcccccc atcgatccca ataacccagc
     3841 agccaactgg cttcatgcgc gctccactcg gaaaaagcgg tgcccctata caaaacacca
     3901 gaccctggaa ctggagaaag agtttctgtt caacatgtac ctcaccaggg accgcaggta
     3961 cgaggtggct cgactgctca acctcaccga gaggcaggtc aagatctggt tccagaaccg
     4021 caggatgaaa atgaagaaaa tcaacaaaga ccgagcaaaa gacgagtagg acccagcttt
     4081 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     4141 gttcgaatac ccatacgacg tcccagacta cgcttagtaa tgattaatta aactagaaat
     4201 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agtaacccac ccaagatctg
     4261 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagccgcgt
     4321 aagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
     4381 agcctagtcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4441 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     4501 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4561 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4621 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     4681 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     4741 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     4801 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     4861 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     4921 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     4981 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     5041 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     5101 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     5161 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     5221 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     5281 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     5341 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     5401 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     5461 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     5521 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccg
     5581 ccatgctagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggatc
     5641 cataacttcg tataatgtat gctatacgaa gttatgccac catgtctaga ctggacaaga
     5701 gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga
     5761 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     5821 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
     5881 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
     5941 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     6001 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     6061 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     6121 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     6181 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     6241 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     6301 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     6361 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     6421 ccgggtaact aagtaaggat ccgcggccgc actagaggaa ttccgcccct cccccccccc
     6481 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     6541 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6601 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6661 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6721 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     6781 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6841 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6901 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6961 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     7021 tgaaaaacac gatgataagc ttaccggtcc accatggtga gcaagggcga ggagctgttc
     7081 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     7141 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     7201 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     7261 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     7321 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     7381 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     7441 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     7501 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     7561 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     7621 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     7681 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     7741 atcactctcg gcatggacga gctgtacaag taagcatgcc cgacggcgag gatctcgtcg
     7801 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7861 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7921 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7981 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
     8041 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     8101 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8161 attgttgttg tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca
     8221 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     8281 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     8341 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     8401 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8461 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8521 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8581 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     8641 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8701 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8761 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     8821 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8881 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8941 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9001 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9061 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9121 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9181 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     9241 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     9301 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9361 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9421 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9481 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9541 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9601 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9661 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9721 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9781 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9841 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9901 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9961 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    10021 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    10081 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    10141 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    10201 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    10261 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10321 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10381 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10441 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10501 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10561 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10621 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10681 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10741 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10801 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10861 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10921 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10981 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    11041 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    11101 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    11161 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    11221 ag
//
