LOCUS       sequence-148186-       12022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of RORA after doxycycline administration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12022)
  AUTHORS   Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, 
            Bernstein ID, Collins JJ, Zon LI, Daley GQ
  TITLE     Induction of multipotential hematopoietic progenitors from human 
            pluripotent stem cells via respecification of lineage-restricted 
            precursors.
  JOURNAL   Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 
            10.1016/j.stem.2013.09.002.
  PUBMED    24094326
REFERENCE   2  (bases 1 to 12022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.09"; journalName: "Cell Stem Cell"; date: 
            "2013-10-3- 3"; volume: "13"; issue: "4"; pages: "459-70"
COMMENT     Sequence Label: pINDUCER21-RORA
FEATURES             Location/Qualifiers
     source          1..12022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(266..271,272..289,290..296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     328..344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     328..344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     394..413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             605..1238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1285..1410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1907..2140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2325..2369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2662..2779
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2834..2852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2876..2894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2918..2936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2960..2978
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3002..3020
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3053..3091
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3088..3112
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3221..3245
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4851..4875)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4928..4954
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5174..6352
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5405..6343
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6300..6320
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6422..6455
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6462..7079,7080..7085,7086..7121,7122..7124,7125..7160,
                     7161..7163,7164..7199,7200..7208)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7265..7838
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7432..7449)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7659..7678
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7854..7856,7857..7859,7860..8573)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7854..7856,7857..7859,7860..8570)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7899..7920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8160..8179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8507..8528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8709..8728
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9016..9604
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9069..9089)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9487..9498)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9679..9912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10031..10049)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10117..10221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10222..10290,10291..11082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10440..10459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11253..11841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11742..11761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11995..12012
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
       61 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
      181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
      361 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
      421 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
      481 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
      541 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
      601 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
      661 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      721 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      781 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      841 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      901 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      961 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     1021 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     1081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1201 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1261 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1321 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1381 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1441 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1501 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1561 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1621 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1681 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1741 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1801 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1861 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1921 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1981 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2041 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2101 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2161 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2221 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2281 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2341 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2401 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2461 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2521 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2581 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2641 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2701 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2761 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2821 gatcctttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2881 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2941 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3001 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     3061 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     3121 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     3181 ccccgaacta gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc
     3241 aggcttgaag gaattcggta ccatggagtc agctccggca gcccccgacc ccgccgccag
     3301 cgagccaggc agcagcggcg cggacgcggc cgccggctcc agggagaccc cgctgaacca
     3361 ggaatccgcc cgcaagagcg agccgcctgc cccggtgcgc agacagagct attccagcac
     3421 cagcagaggt atctcagtaa cgaagaagac acatacatct caaattgaaa ttattccatg
     3481 caagatctgt ggagacaaat catcaggaat ccattatggt gtcattacat gtgaaggctg
     3541 caagggcttt ttcaggagaa gtcagcaaag caatgccacc tactcctgtc ctcgtcagaa
     3601 gaactgtttg attgatcgaa ccagtagaaa ccgctgccaa cactgtcgat tacagaaatg
     3661 ccttgccgta gggatgtctc gagatgctgt aaaatttggc cgaatgtcaa aaaagcagag
     3721 agacagcttg tatgcagaag tacagaaaca ccggatgcag cagcagcagc gcgaccacca
     3781 gcagcagcct ggagaggctg agccgctgac gcccacctac aacatctcgg ccaacgggct
     3841 gacggaactt cacgacgacc tcagtaacta cattgacggg cacacccctg aggggagtaa
     3901 ggcagactcc gccgtcagca gcttctacct ggacatacag ccttccccag accagtcagg
     3961 tcttgatatc aatggaatca aaccagaacc aatatgtgac tacacaccag catcaggctt
     4021 ctttccctac tgttcgttca ccaacggcga gacttcccca actgtgtcca tggcagaatt
     4081 agaacacctt gcacagaata tatctaaatc gcatctggaa acctgccaat acttgagaga
     4141 agagctccag cagataacgt ggcagacctt tttacaggaa gaaattgaga actatcaaaa
     4201 caagcagcgg gaggtgatgt ggcaattgtg tgccatcaaa attacagaag ctatacagta
     4261 tgtggtggag tttgccaaac gcattgatgg atttatggaa ctgtgtcaaa atgatcaaat
     4321 tgtgcttcta aaagcaggtt ctctagaggt ggtgtttatc agaatgtgcc gtgcctttga
     4381 ctctcagaac aacaccgtgt actttgatgg gaagtatgcc agccccgacg tcttcaaatc
     4441 cttaggttgt gaagacttta ttagctttgt gtttgaattt ggaaagagtt tatgttctat
     4501 gcacctgact gaagatgaaa ttgcattatt ttctgcattt gtactgatgt cagcagatcg
     4561 ctcatggctg caagaaaagg taaaaattga aaaactgcaa cagaaaattc agctagctct
     4621 tcaacacgtc ctacagaaga atcaccgaga agatggaata ctaacaaagt taatatgcaa
     4681 ggtgtctaca ttaagagcct tatgtggacg acatacagaa aagctaatgg catttaaagc
     4741 aatataccca gacattgtgc gacttcattt tcctccatta tacaaggagt tgttcacttc
     4801 agaatttgag ccagcaatgc aaattgatgg gtagctcgag tgcggccgca acccagcttt
     4861 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     4921 gttcgaatac ccatacgacg tcccagacta cgcttagtaa tgattaatta aactagaaat
     4981 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agtaacccac ccaagatctg
     5041 gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagccgcgt
     5101 aagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
     5161 agcctagtcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5221 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     5281 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     5341 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     5401 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     5461 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     5521 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     5581 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     5641 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     5701 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     5761 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     5821 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     5881 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     5941 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     6001 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     6061 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     6121 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     6181 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     6241 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     6301 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccg
     6361 ccatgctagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggatc
     6421 cataacttcg tataatgtat gctatacgaa gttatgccac catgtctaga ctggacaaga
     6481 gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga
     6541 caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga
     6601 acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact
     6661 tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc
     6721 gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag
     6781 agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc
     6841 tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg
     6901 aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc
     6961 ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc
     7021 ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg
     7081 ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg
     7141 actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc
     7201 ccgggtaact aagtaaggat ccgcggccgc actagaggaa ttccgcccct ctcccccccc
     7261 cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     7321 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     7381 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     7441 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     7501 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     7561 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     7621 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     7681 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     7741 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     7801 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttaccggtcc accatggtga
     7861 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7921 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7981 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     8041 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     8101 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8161 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8221 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8281 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     8341 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     8401 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     8461 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     8521 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagcatgcc
     8581 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8641 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8701 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8761 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8821 tcttgacgag ttcttctgat gtacaagtaa agcggccgcg actctagatc ataatcagcc
     8881 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     8941 tgaaacataa aatgaatgca attgttgttg tttagtccct cccaattcga tatcaagctt
     9001 atcgcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     9061 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     9121 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     9181 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     9241 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     9301 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9361 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     9421 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9481 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9541 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9601 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9661 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9721 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9781 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9841 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9901 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9961 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
    10021 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    10081 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10141 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10201 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10261 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10321 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10381 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10441 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
    10501 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10561 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10621 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10681 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10741 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10801 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10861 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10921 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10981 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    11041 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11101 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11161 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11941 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    12001 tcagtgagcg aggaagcgga ag
//
