LOCUS       sequence-148189-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ffDronpa E. coli strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, 
            van Meervelt L, Hofkens J, Dedecker P
  TITLE     Green-to-red photoconvertible Dronpa mutant for multimodal 
            super-resolution fluorescence microscopy.
  JOURNAL   ACS Nano. 2014 Feb 25;8(2):1664-73. doi: 10.1021/nn4060144. Epub 
            2014 Jan 21.
  PUBMED    24410188
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn4060144";
            journalName: "ACS Nano"; date: "2014-02-25- 25"; volume: "8"; issue:
            "2"; pages: "1664-73"
COMMENT     Sequence Label: pRSETb-ffDronpa
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..710)
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTAFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
     CDS             complement(join(717..731,732..740))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(744..776)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(780..797)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggctttgtt agcagccgga tcaagcttcg aattcttact tggcctgcct cggcagctca
       61 gaatgcgctt cggcatgctc atgcagatta acattactgt aatctttgtc gtggctttta
      121 atctcaatgt ggtggtccac aaagtgatag tctggcaact ggacaacctt cttagcttta
      181 taagtagttt tgaagtcaca tcggtaatgg ccacctcctt caagcgacag agccatgtta
      241 acatcaccct tcagcactcc atcacgcaca tacaatttct cagtggatgg ctcccatttc
      301 acagtcctct tctgcataac tggaccattg gcaggaaagt tcacaccatc aaatcgaatt
      361 tcatagatat aacagtcacc atccagggtt atgtcgtttg tcgcgttaca aatgcccccg
      421 tcttcgtaat tcatgcttcg ttcccaagag tagccctcag gaaacgactg cttgaaatag
      481 tctactatat tttctgggta tttggcgaat accctgttgc cgtaacagaa cgcagttgtc
      541 aagatgtcat aggcgaaagg cagaggtccg ccttctttga ctttaaggtc catactctgt
      601 tttccctcga aaggcttccc aaggccaact ccttcaatcg cgaacgggtg tccatttaca
      661 gcgccttcca tacgcagctt gatcttcatg tctggtttaa tcacactcat cggatcctta
      721 tcgtcatcgt cgtacagatc ccgacccatt tgctgtccac cagtcatgct agccatacca
      781 tgatgatgat gatgatgaga accc
//
