LOCUS       sequence-148196-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Sidik SM, Hortua Triana MA, Paul AS, El Bakkouri M, Hackett CG, Tran
            F, Westwood NJ, Hui R, Zuercher WJ, Duraisingh MT, Moreno SN, 
            Lourido S
  TITLE     Using a Genetically Encoded Sensor to Identify Inhibitors of 
            Toxoplasma gondii Ca2+ Signaling.
  JOURNAL   J Biol Chem. 2016 Apr 29;291(18):9566-80. doi: 
            10.1074/jbc.M115.703546. Epub 2016 Mar 1.
  PUBMED    26933036
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.703546"; journalName: "J Biol Chem"; date: 
            "2016-04-29- 29"; volume: "291"; issue: "18"; pages: "9566-80"
COMMENT     Sequence Label: pGCaMP5-DHFR
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             847..864
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggtatcgata agcttttaca tccgttgcct tttccacggt ccgtgatttc atgtgcgtgc
       61 agcttcaaag actggtcgtt gcgactaata anactgcagt gacaggtcga atggtgggca
      121 ccttgctgat gactatctac tgcaaagtct gagacaacga acgaaacttc ccacacgagg
      181 catttgaaac tgacggtgtc taggtaatat gcactgcaag acacggtact ggggcctcgc
      241 tgaattaggg gccgatctcg ttgccctatc agtgctcaca gtgccgcaac gtaacaccag
      301 ggcaggttct tgacagtggc aacaatgtgc gacgggcgtg tgaacgtttc gtagtcatag
      361 cgctagcacg tacctagcca catggtcgtg aggagcttta ccatgcgtct agaaggtgga
      421 tgcgggacac gccttcctgg cctttggctc ccgagacgcg tgttctaacc acaaaccttg
      481 agacgcgtgt tccaaccacg caccctgaca cgcgtgttcc aaccacgcac cctgagacgc
      541 gtgttctaac cacgcaccct gagacgcgtg ttctaaccac gcaccctgag acgcgtgttc
      601 tgccgcacaa tgtgcacctg taggaagctg tagtcactgc tgattctcac tgttctcggc
      661 aagggccgac gaccggagta cagtttttgt gggcagagcc gttgtgcagc tttccgttct
      721 tctcggttgt gtcacatgtg tcattgtcgt gtaaacacac ggttgtatgt cggtttcgct
      781 gcaccacttc attatttctt ctggtttttt gacgagtatg catccttttt cgacaaaatg
      841 ggttctcatc atcatcatca tcatggtatg gctagcatga ctgg
//
