LOCUS       sequence-148197-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Sidik SM, Hortua Triana MA, Paul AS, El Bakkouri M, Hackett CG, Tran
            F, Westwood NJ, Hui R, Zuercher WJ, Duraisingh MT, Moreno SN, 
            Lourido S
  TITLE     Using a Genetically Encoded Sensor to Identify Inhibitors of 
            Toxoplasma gondii Ca2+ Signaling.
  JOURNAL   J Biol Chem. 2016 Apr 29;291(18):9566-80. doi: 
            10.1074/jbc.M115.703546. Epub 2016 Mar 1.
  PUBMED    26933036
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.703546"; journalName: "J Biol Chem"; date: 
            "2016-04-29- 29"; volume: "291"; issue: "18"; pages: "9566-80"
COMMENT     Sequence Label: pGCaMP5
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(348..791)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc tcgggggggc aagaattgtg ttaaccggtt
       61 cgactaaaac aactgatagc tgtcttgtgt attgacccat gtggcgcgtg tgtcgacggc
      121 gatcaacatc agccaggtcc gccacacatc cgttcagaga ttcggaccca gtcataaact
      181 ttcgctgctt gctgcgaaac actcctattt agagtcggcc ggtttttgtg gatgtgacac
      241 ctgcaagcca caacggaaca actcagtttc tttataatgg ggcacatgct gcacgaagtg
      301 tgtttccttt gtcgatttga gaagtgagca caacggtgat taattaatca cttcgctgtc
      361 atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc tgcttccctg
      421 atcatttcat caacctcttc atctgttaac ttctctccaa ggtttgtcat cacgtggcga
      481 agctctgctg cactgatgta gccattgcca tccttatcaa acacacggaa cgcttctcta
      541 atttcttctt cactgtctgt gtatttcatt tttcttgcca tcattgtcag gaactcaggg
      601 aagtcgattg tgccgtcacc gtcggcatct acttcattga tcatgtcctg cagctctgct
      661 tctgtggggt tctgccccag agaccgcatc accgtcccca gctccttggt tgttattgtc
      721 ccatccccgt ccttgtcaaa tagggagaaa gcctctttaa attctgcgat ctgctcttca
      781 gtcagttggt ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
      841 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
      901 gcgcgggtct tgta
//
