LOCUS       sequence-148199-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Sidik SM, Hortua Triana MA, Paul AS, El Bakkouri M, Hackett CG, Tran
            F, Westwood NJ, Hui R, Zuercher WJ, Duraisingh MT, Moreno SN, 
            Lourido S
  TITLE     Using a Genetically Encoded Sensor to Identify Inhibitors of 
            Toxoplasma gondii Ca2+ Signaling.
  JOURNAL   J Biol Chem. 2016 Apr 29;291(18):9566-80. doi: 
            10.1074/jbc.M115.703546. Epub 2016 Mar 1.
  PUBMED    26933036
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.703546"; journalName: "J Biol Chem"; date: 
            "2016-04-29- 29"; volume: "291"; issue: "18"; pages: "9566-80"
COMMENT     Sequence Label: pGCaMP5-DHFR
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggcggccgct ctagaactag tggatcgatc ccccgggctg caggaattcc atcctgcaag
       61 tgcatagaag gaaagttgtc tgctgtcgtg ggcagacagc aacagtccag cactctagcg
      121 gcatacagaa cgataacgca ttcacgagtg gatacacgca catctgcgtc acccgcaact
      181 cgctttcgtt ctgattgaca aaaagaaaac aaggcgaggt gagactgtgt gaaatgccac
      241 atgaagagtc atcccttttc ttcgataaag gacacagggg tctctggcac cccctcgtca
      301 gctctctccg acccgaggca ctctccctga tccctccgaa aagagaggaa aacgagagac
      361 gggcagcttc tgtatttccg ctagacagcc atctccatct ggattcgtcc gtgcgggacg
      421 tagcccacga cctcaaaatc ctcggcggtg aaatcgtcga tttccttgat gcgttccttg
      481 ttgaggatgt tcacaatggg gaacggtctc ggttctctcc gcagctgctc ttttaaagcc
      541 tcgacatggt tcgtgtagac atgcgtgttc cccatgaagt gaatgaactc cttaggtttt
      601 aggttgcaga cgtgtgcaac catgagcgtc aaaagcgaat aggaagcgat gttgaagggg
      661 acgccgaggc cgacatcgca cgaccgctga tacatgatgc acgacagctc cttctggtcg
      721 ttcacgtaga actggcacaa caagtgacaa ggcggcagcg ccatttcgtc cagcgctgca
      781 ggattccagg cagtcatgag catgcgacga tctgttggat tcgttctcag catctggatc
      841 acattcttca gctggtcgac gccctgccct gtgtagtctg tgtgcatgtc tttgtatgcc
      901 gcgccgaagt gtctccactg gaagccgtag cccgggccga tgtctccgac ctctcggtgg
      961 ggg
//
