LOCUS       sequence-148205-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses pcDronpa2 in E. coli strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Moeyaert B, Nguyen Bich N, De Zitter E, Rocha S, Clays K, Mizuno H, 
            van Meervelt L, Hofkens J, Dedecker P
  TITLE     Green-to-red photoconvertible Dronpa mutant for multimodal 
            super-resolution fluorescence microscopy.
  JOURNAL   ACS Nano. 2014 Feb 25;8(2):1664-73. doi: 10.1021/nn4060144. Epub 
            2014 Jan 21.
  PUBMED    24410188
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nn4060144";
            journalName: "ACS Nano"; date: "2014-02-25- 25"; volume: "8"; issue:
            "2"; pages: "1664-73"
COMMENT     Sequence Label: pRSETb-pcDronpa2
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             55..87
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(91..99,100..114)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca tggtatggct
       61 agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga taaggatccg
      121 atgagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc cgtaaatgga
      181 cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa acagagtatg
      241 gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt gacaactgcg
      301 ttccattacg gcaacagggt attcgccaaa tacccagaaa atatagtaga ctatttcaag
      361 cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga gttacgaaga cgggggcatt
      421 tgtatcgcga caaacgacat aaccctggat ggtgactgtt atatcaacga aattcgattt
      481 gatggtgtga actttcctgc caatggtcca gttatgcaga agaggactgt gaaatgggag
      541 ccatccactg agaaattgta tgtgcgtgat ggagtgctga agggtgatgt taacatggct
      601 ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta taaagctaag
      661 aaggttgtcc agttgccaga ctaccacttt gtggaccacc acattgagat taaaagccac
      721 gacaaagatt acagtaatgt taatctgcac gagcatgccg aagcgcattc tggg
//
