LOCUS       sequence-148217-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rsGreen1 in E. coli strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Duwe S, De Zitter E, Gielen V, Moeyaert B, Vandenberg W, Grotjohann 
            T, Clays K, Jakobs S, Van Meervelt L, Dedecker P
  TITLE     Expression-Enhanced Fluorescent Proteins Based on Enhanced Green 
            Fluorescent Protein for Super-resolution Microscopy.
  JOURNAL   ACS Nano. 2015 Oct 27;9(10):9528-41. doi: 10.1021/acsnano.5b04129. 
            Epub 2015 Sep 9.
  PUBMED    26308583
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04129"; journalName: "ACS Nano"; date: 
            "2015-10-27- 27"; volume: "9"; issue: "10"; pages: "9528-41"
COMMENT     Sequence Label: pRSETb-rsGreen1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..755)
                     /codon_start=1
                     /product="reversibly switchable enhanced green fluorescent 
                     protein (Grotjohann et al., 2011)"
                     /label=rsEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTM
                     KFICTTGKLPVPWPTLVTTLTYGVLCFARYPDHMKQHDFFKSAMPEGYVQERTISFEDD
                     GYYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHDAYITADKQKNGIR
                     SNFKIRLNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQNKLSKDPNEKRDHMVLL
                     EFVTASGITLGMDELYK"
     primer_bind     complement(81..102)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     689..710
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(join(762..776,777..785))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(789..821)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(825..842)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt agcagccgga tcaagcttcg aattcttact tgtacagctc gtccatgccg
       61 agagtgatcc cggaggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      121 gggtctttgc tcagcttatt ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
      181 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
      241 acgttgaggc ggatcttgaa gttagacctg atgccgttct tctgcttgtc ggccgtgata
      301 taggcgtcgt ggctgttgaa gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      361 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      421 tcggcgcggg tcttgtaata gccgtcgtcc tcgaagctga tggtgcgctc ctggacgtag
      481 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
      541 aagcacagca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      601 ccggtggtgc agatgaactt catggtcagc ttgccgttgg tggcatcgcc ctcgccctcg
      661 ccgcgcacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      721 accccggtga acagctcctc gcccttgctc accatcggat ccttatcgtc atcgtcgtac
      781 agatcccgac ccatttgctg tccaccagtc atgctagcca taccatgatg atgatgatga
      841 tgagaacccc gcatatgtat atctccttct taaagt
//
