LOCUS       sequence-148219-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses rsGreenF in E. coli strains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Duwe S, De Zitter E, Gielen V, Moeyaert B, Vandenberg W, Grotjohann 
            T, Clays K, Jakobs S, Van Meervelt L, Dedecker P
  TITLE     Expression-Enhanced Fluorescent Proteins Based on Enhanced Green 
            Fluorescent Protein for Super-resolution Microscopy.
  JOURNAL   ACS Nano. 2015 Oct 27;9(10):9528-41. doi: 10.1021/acsnano.5b04129. 
            Epub 2015 Sep 9.
  PUBMED    26308583
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsnano.5b04129"; journalName: "ACS Nano"; date: 
            "2015-10-27- 27"; volume: "9"; issue: "10"; pages: "9528-41"
COMMENT     Sequence Label: pRSETb-rsGreenF
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..749)
                     /codon_start=1
                     /product="reversibly switchable enhanced green fluorescent 
                     protein (Grotjohann et al., 2011)"
                     /label=rsEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTM
                     KFICTTGKLPVPWPTLVTTLTYGVLCFARYPDHMKQHDFFKSAMPEGYVQERTISFEDD
                     GYYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLNSHNAYITADKQKNGIR
                     SNFKIRLNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQNKLSKDPNEKRDHMVLL
                     EFVTASGITLGMDELYK"
     primer_bind     complement(75..96)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     683..704
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(join(756..770,771..779))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(783..815)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(819..836)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggaatcaagc ttcgaattct tacttgtaca gctcgtccat gccgagagtg
       61 atcccggagg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      121 ttgctcagct tattctgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      181 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgacgttg
      241 aggcggatct tgaagttaga cctgatgccg ttcttctgct tgtcggccgt gatataggcg
      301 ttgtggctgt tgaggttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
      361 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
      421 cgggtcttgt aatagccgtc gtcctcgaag ctgatggtgc gctcctggac gtagccttcg
      481 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac
      541 agcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcactggcag cttgccggtg
      601 gtgcagatga acttcatggt cagcttgccg ttggtggcat cgccctcgcc ctcgccgcgc
      661 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      721 gtgaacagct cctcgccctt gctcaccatc ggatccttat cgtcatcgtc gtacagatcc
      781 cgacccattt gctgtccacc agtcatgcta gccataccat gatgatgatg atgatgagaa
      841 cc
//
