LOCUS       sequence-148220-       12190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Wei Q, Adelstein RS
  TITLE     Conditional expression of a truncated fragment of nonmuscle myosin 
            II-A alters cell shape but not cytokinesis in HeLa cells.
  JOURNAL   Mol Biol Cell. 2000 Oct . 11(10):3617-27.
  PUBMED    11029059
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2000 Oct . 11(10):3617-27."
COMMENT     Sequence Label: CMV-GFP-NMHC II-B
FEATURES             Location/Qualifiers
     source          1..12190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     526..546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      614..623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(620..622,623..625,626..1336)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(620..622,623..625,626..1336)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(665..686)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(926..945)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1273..1294
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8818..8834)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8818..8834)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    8985..9106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9022..9041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9076..9095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9113..9568)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9250..9271)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9462..9481
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9595..9699
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9701..10058
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9701..9892
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9724..9744)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9909..10044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10093..10887
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10093..10887
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10147..10166)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10757..10776
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11075..11094)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11119..11166
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11495..12083
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11984..12003
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      661 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      721 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      781 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
      841 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      901 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      961 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1021 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1081 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1141 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1201 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     1261 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1321 ggacgagctg tacaagtact cagatctcga gctcaagctt cgaattccaa tggcgcagag
     1381 aactggactc gaggatccag agaggtatct ctttgtggac agggctgtca tctacaaccc
     1441 tgccactcaa gctgattgga cagctaaaaa gctagtgtgg attccatcag aacgccatgg
     1501 ttttgaggca gctagtatca aagaagaacg gggagatgaa gttatggtgg agttggcaga
     1561 gaatggaaag aaagcaatgg tcaacaaaga tgatattcag aagatgaacc cacctaagtt
     1621 ttccaaggtg gaggatatgg cagaattgac atgcttgaat gaagcttccg ttttacataa
     1681 tctgaaggat cgctactatt caggactaat ctatacttat tctggactct tctgtgtagt
     1741 tataaaccct tacaagaatc ttccaattta ctctgagaat attattgaaa tgtacagagg
     1801 gaagaagcgt catgagatgc ctccacacat ctatgctata tctgaatctg cttacagatg
     1861 catgcttcaa gatcgtgagg accagtcaat tctttgcacg ggtgagtcag gtgctgggaa
     1921 gacagaaaat acaaagaaag ttattcagta ccttgcccat gttgcttctt cacataaagg
     1981 aagaaaggac cataatattc ctggggaact tgaacggcag cttttgcaag caaatccaat
     2041 tctggaatca tttggaaatg cgaagactgt gaaaaatgat aactcatctc gttttggcaa
     2101 atttattcgg atcaactttg atgtaactgg ctatatcgtt ggggccaaca ttgaaacata
     2161 ccttctggaa aagtctcgtg ctgttcgtca agcaaaagat gaacgtactt ttcatatctt
     2221 ttaccagttg ttatctggag caggagaaca cctaaagtct gatttgcttc ttgaaggatt
     2281 taataactac aggtttctct ccaatggcta tattcctatt ccgggacagc aagacaaaga
     2341 taatttccag gagaccatgg aagcaatgca cataatgggc ttctcccatg aagagattct
     2401 gtcaatgctt aaagtagtat cttcagtgct acagtttgga aatatttctt tcaaaaagga
     2461 gagaaatact gatcaagctt ccatgccaga aaatacagtt gcgcagaagc tctgccatct
     2521 tcttgggatg aatgtgatgg agtttactcg ggccatcctg actccccgga tcaaggtcgg
     2581 ccgagactat gtgcaaaaag cccagaccaa agaacaggca gattttgcag tagaagcatt
     2641 ggcaaaagct acctatgagc ggctctttcg ctggctcgtt catcgcatca ataaagctct
     2701 ggataggacc aaacgtcagg gagcatcttt cattggaatc ctggatattg ctggatttga
     2761 aatttttgag ctgaactcct ttgaacaact ttgcatcaac tacaccaatg agaagctgca
     2821 gcagctgttc aaccacacca tgtttatcct agaacaagag gaataccagc gcgaaggcat
     2881 cgagtggaac ttcatcgatt tcgggctgga tctgcagcca tgcatcgacc taatagagag
     2941 acctgcgaac cctcctggtg tactggccct tttggatgaa gaatgctggt tccctaaagc
     3001 cacagataaa acctttgttg aaaaactggt tcaagagcaa ggttcccact ccaagtttca
     3061 gaaacctcga caattaaaag acaaagctga tttttgcatt atacattatg cagggaaggt
     3121 ggactataag gcagatgagt ggctgatgaa gaatatggac cccctgaatg acaacgtggc
     3181 cacccttttg caccagtcat cagacagatt tgtggcagag ctttggaaag atgtggaccg
     3241 tatcgtgggt ctggatcaag tcactggtat gactgagaca gcttttggct ccgcatataa
     3301 aaccaagaag ggcatgtttc gtaccgttgg gcaactctac aaagaatctc tcaccaagct
     3361 gatggcaact ctccgaaaca ccaaccctaa ctttgttcgt tgtatcattc caaatcacga
     3421 gaagagggct ggaaaattgg atccacacct agtcctagat cagcttcgct gtaatggtgt
     3481 cctggaaggg atccgaatct gtcgccaggg cttccctaac cgaatagttt tccaggaatt
     3541 cagacagaga tatgagatcc taactccaaa tgctattcct aaaggtttta tggatggtaa
     3601 acaggcctgt gaacgaatga tccgggcttt agaattggac ccaaacttgt acagaattgg
     3661 acagagcaag atatttttca gagctggagt tctggcacac ttagaggaag aaagagattt
     3721 aaaaatcacc gatatcatta tcttcttcca ggccgtttgc agaggttacc tggccagaaa
     3781 ggcctttgcc aagaagcagc agcaactaag tgccttaaag gtcttgcagc ggaactgtgc
     3841 cgcgtacctg aaattacggc actggcagtg gtggcgagtc ttcacaaagg tgaagccgct
     3901 tctacaagtg actcgccagg aggaagaact tcaggccaaa gatgaagagc tgttgaaggt
     3961 gaaggagaag cagacaaagg tggaaggaga gctggaggag atggagcgga agcaccagca
     4021 gcttttagaa gagaagaata tccttgcaga acaactacaa gcagagactg agctctttgc
     4081 tgaagcagaa gagatgaggg caagacttgc tgctaaaaag caggaattag aagagattct
     4141 acatgacttg gagtctaggg ttgaagaaga agaagaaaga aaccaaatcc tccaaaatga
     4201 aaagaaaaaa atgcaagcac atattcagga cctggaagaa cagctagacg aggaggaagg
     4261 ggctcggcaa aagctgcagc tggaaaaggt gacagcagag gccaagatca agaagatgga
     4321 agaggagatt ctgcttctcg aggaccaaaa ttccaagttc atcaaagaaa agaaactcat
     4381 ggaagatcgc attgctgagt gttcctctca gctggctgaa gaggaagaaa aggcgaaaaa
     4441 cttggccaaa atcaggaata agcaagaagt gatgatctca gatctagaag aacgcttaaa
     4501 gaaggaagaa aagactcgtc aggaactgga aaaggccaaa agaaaactcg acggggagac
     4561 gaccgacctg caggaccaga tcgcagagct gcaggcgcag attgatgagc tcaagctgca
     4621 gctggccaag aaggaggagg agctgcaggg cgcactggcc agaggtgatg atgaaacact
     4681 ccataagaac aatgccctta aagttgtgcg agagctacaa gcccaaattg ctgaacttca
     4741 ggaagacttt gaatccgaga aggcttcacg gaacaaggcc gaaaagcaga aaagggactt
     4801 gagtgaggaa ctggaagctc tgaaaacaga gctggaggac acgctggaca ccacggcagc
     4861 ccagcaggaa ctacgtacaa aacgtgaaca agaagtggca gagctgaaga aagctcttga
     4921 ggaggaaact aagaaccatg aagctcaaat ccaggacatg agacaaagac acgcaacagc
     4981 cctggaggag ctctcagagc agctggaaca ggccaagcgg ttcaaagcaa atctagagaa
     5041 gaacaagcag ggcctggaga cagataacaa ggagctggcg tgtgaggtga aggtcctgca
     5101 gcaggtcaag gctgagtctg agcacaagag gaagaagctc gacgcgcagg tccaggagct
     5161 ccatgccaag gtctctgaag gcgacaggct cagggtggag ctggcggaga aagcaagtaa
     5221 gctgcagaat gagctagata atgtctccac ccttctggaa gaagcagaga agaagggtat
     5281 taaatttgct aaggatgcag ctagtcttga gtctcaacta caggatacac aggagcttct
     5341 tcaggaggag acacgccaga aactaaacct gagcagtcgg atccggcagc tggaagagga
     5401 gaagaacagt cttcaggagc agcaggagga ggaggaggag gccaggaaga acctggagaa
     5461 gcaagtgctg gccctgcagt cccagttggc tgataccaag aagaaagtag atgacgacct
     5521 gggaacaatt gaaagtctgg aagaagccaa gaagaagctt ctgaaggacg cggaggccct
     5581 gagccagcgc ctggaggaga aggcactggc gtatgacaaa ctggagaaga ccaagaaccg
     5641 cctgcagcag gagctggacg acctcacggt ggacctggac caccagcgcc aggtcgcctc
     5701 caacttggag aagaagcaga agaagtttga ccagctgtta gcagaagaga agagcatctc
     5761 tgctcgctat gccgaagagc gggaccgggc cgaagccgag gccagagaga aagaaaccaa
     5821 agccctgtca ctggcccggg ccctcgagga agccctggag gccaaggagg agtttgagag
     5881 gcagaacaag cagctccgag cagacatgga agacctcatg agctccaaag atgatgtggg
     5941 aaaaaacgtt cacgaacttg aaaaatccaa acgggcccta gagcagcagg tggaggaaat
     6001 gaggacccag ctggaggagc tggaagacga actccaggcc acggaagatg ccaagcttcg
     6061 tctggaggtc aacatgcagg ccatgaaggc gcagttcgag agagacctgc aaaccaggga
     6121 tgagcagaat gaagagaaga agcggctgct gatcaaacag gtgcgggagc tcgaggcgga
     6181 gctggaggat gagaggaaac agcgggcgct tgctgtagct tcaaagaaaa agatggagat
     6241 agacctgaag gacctcgaag cccaaatcga ggctgcgaac aaagctcggg atgaggtgat
     6301 taagcagctc cgcaagctcc aggctcagat gaaggattac caacgtgaat tagaagaagc
     6361 tcgtgcatcc agagatgaga tttttgctca atccaaagag agtgaaaaga aattgaagag
     6421 tctggaagca gaaatccttc aattgcagga ggaacttgcc tcatctgagc gagcccgccg
     6481 acacgccgag caggagagag atgagctggc ggacgagatc accaacagcg cctctggcaa
     6541 gtccgcgctg ctggatgaga agcggcgtct ggaagctcgg atcgcacagc tggaggagga
     6601 gctggaagag gagcagagca acatggagct gctcaacgac cgcttccgca agaccactct
     6661 acaggtggac acactgaacg ccgagctagc agccgagcgc agcgccgccc agaagagtga
     6721 caatgcacgc cagcaactgg agcggcagaa caaggagctg aaggccaagc tgcaggaact
     6781 cgagggtgct gtcaagtcta agttcaaggc caccatctca gccctggagg ccaagattgg
     6841 gcagctggag gagcagcttg agcaggaagc caaggaacga gcagccgcca acaaattagt
     6901 ccgtcgcact gagaagaagc tgaaagaaat cttcatgcag gttgaggatg agcgtcgaca
     6961 cgcggaccag tataaagagc agatggagaa ggccaacgct cggatgaagc agcttaaacg
     7021 ccagctggag gaagcagaag aagaagcgac gcgtgccaac gcatctcggc gtaaactcca
     7081 gcgggaactg gatgatgcca ccgaggccaa cgagggcctg agccgcgagg tcagcaccct
     7141 gaagaaccgg ctgaggcggg gtggccccat cagcttctct tccagccgat ctggccggcg
     7201 ccagctgcac cttgaaggag cttccctgga gctctccgac gatgacacag aaagtaagac
     7261 cagtgatgtc aacgagacgc agccacccca gtcagagtaa agttgcagga agccagagga
     7321 ggcaatacag tgggacagtt aggaatgcac ccggggcctc ctgcagattt cggaaattgg
     7381 caagctacgg gattccttcc tgaaagatca actgtgtctt aaggctctcc agcctatgca
     7441 tactgtatcc tgcttcagac ttaggtacaa ttgctcccct ttttatatat agacacacac
     7501 aggacacata tattaaacag attgtttcat cattgcatct attttccata tagtcatcaa
     7561 gagaccattt tataaaacat ggtaagaccc tttttaaaac aaactccagg cccttggttg
     7621 cgggtcgctg ggttattggg gcagcgccgt ggtcgtcact cagtcgctct gcatgctctc
     7681 tgtcatacag acaggtaacc tagttctgtg ttcacgtggc ccccgacgac tcagccacat
     7741 caagtctcct agaccactgt ggactctaaa ctgcacttgt ctctctcatt tccttcaaat
     7801 aatgatcaat gctatttcag tgagcaaact gtgaaagggg ctttggaaag agtaggaggg
     7861 gtgggctgga tcggaagcaa cacccatttg gggttaccat gtccatcccc caaggggggc
     7921 cctccccctc gagtcgatgg tgtcccgcat ctactcatgt gaactggcct tggcgagggc
     7981 tggtctgtgc atagaaggga tagtggccac actgcagctg aggccccagg tggcagccat
     8041 ggatcatgta gacttccaga tggtctcccg aaccgcctgg ctctgccggc gccctcctca
     8101 cgtcaggagc aagcagccgt ggacccctaa gccgagctgg tggaaggccc ctccccgtcg
     8161 ccagccgggc cctcatgctg accttgcaaa ttcagccgct gctttgagcc caaaatggga
     8221 atattggttt tgtgtccgag gcttgttcca agtttgtcaa tgaggtttat ggagcctcca
     8281 gaacagatgc catcttcctg aatgttgaca tgccagtggg tgtgactcct tcatttttcc
     8341 ttctcccttc cctttggaca gtgttacagt gaacacttag catcctgttt ttggttggta
     8401 gttaagcaaa ctgacattac ggaaagtgcc ttagacacta cagtactaag acaatgttga
     8461 atatatcatt cgcctctata acaatttaat gtattcagtt ttgactgtgc ttcatatcat
     8521 gtacctctct agtcaaagtg gtattacaga cattcagtga caatgaatca gtgttaattc
     8581 taaatccttg atcctctgca atgtgcttga aaacacaaac cttttgggtt aaaagcttta
     8641 acatctatta ggaagaattt gtcctgtggg tttggaatct tggattttcc ccctttatga
     8701 actgtactgg ctgttgacca ccagacacct gaccgcaaat atcttttctt gtattcccat
     8761 atttctagac aatgattttt gtaagacaat gcggccgcga attcctgcag cccgggggat
     8821 ccactagttc tagagcggcc gccaccgcgg gcccgggatc caccggatct agataactga
     8881 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     8941 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     9121 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9181 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9241 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9301 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     9361 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     9421 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     9481 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     9541 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9661 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     9721 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     9781 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9841 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9901 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9961 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10021 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
    10081 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    10141 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    10201 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
    10261 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
    10321 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
    10381 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
    10441 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
    10501 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
    10561 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
    10621 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
    10681 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
    10741 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    10801 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    10861 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
    10921 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
    10981 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
    11041 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
    11101 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
    11161 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
    11221 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
    11281 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
    11341 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11401 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11461 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11521 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11581 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11641 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11701 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    11761 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    11821 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11881 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11941 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    12001 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    12061 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12121 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12181 gtattaccgc
//
