LOCUS       sequence-148227-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  acta2 promoter for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Whitesell TR, Kennedy RM, Carter AD, Rollins EL, Georgijevic S, 
            Santoro MM, Childs SJ
  TITLE     An alpha-smooth muscle actin (acta2/alphasma) zebrafish transgenic 
            line marking vascular mural cells and visceral smooth muscle cells.
  JOURNAL   PLoS One. 2014 Mar 3;9(3):e90590. doi: 10.1371/journal.pone.0090590.
            eCollection 2014.
  PUBMED    24594685
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-3"; volume: "9(3):e90590. doi"; pages: " 
            10.1371/journal.pone.0090590. eCollection 2014"
COMMENT     Sequence Label: p5E-acta2
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    5..100
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gctccaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag
       61 caatgctttt ttataatgcc aactttgtat agaaaagttg gccacctagc ttctctcacc
      121 tccttctctt tctattttgc aagctttatc tgattactct tgaaactcgc tgtgtaaagg
      181 gtcaaagaag tgctgttgtt gttgttgttg ttttttgcac aggtgtaagc tggctttaaa
      241 tattttggct ggttagagtg ttttctgaga aagatttgat ctacatttct gtaaatgtga
      301 ttgtgaggat tgattttcct agagacgtgc cttctgaaag atacattttc tacctatctt
      361 ccttttcttt aaagaagcac ttaaagtttt aaagaacaat tctgcatggt tgtgtctaaa
      421 gcatccatat tttagtatct ttattgtgat tttatagtaa aatctctgtg ggtgtgagtg
      481 ttttttaaag agaggttctg aatggctgtt gaagcctgtc tgatttgtta ggtttttgac
      541 taaatatggt tcttctggac cgctgaccag tcccctcttt tcctcaccca ctctctcttt
      601 tttcataaac tattaggcag agagagactg agagagggag agaccaaaga cagacatgct
      661 tccccatcat ttccaaatta ggaagttttc ccatggtgct ctcagcaagg gtctttagga
      721 aatgtgtgta catatacatg agtgtgtgtg cacatataga aaagtacatt tttcttcgta
      781 atgtattggt attgttataa ttgtgttact actaactttt taggatatat aacatatatt
      841 tagaaa
//
