LOCUS       sequence-148230-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  kdrl promoter for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Choi J, Dong L, Ahn J, Dao D, Hammerschmidt M, Chen JN
  TITLE     FoxH1 negatively modulates flk1 gene expression and vascular 
            formation in zebrafish.
  JOURNAL   Dev Biol. 2007 Apr 15;304(2):735-44. Epub 2007 Jan 20.
  PUBMED    17306248
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2007-04-15"; volume: "304(2)"; pages: "735-44. Epub 2007 Jan 
            20"
COMMENT     Sequence Label: p5E-kdrl
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     95..114
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        95..113
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     138..154
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     139..155
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 tttttataat gccaactttg tatagaaaag ttggtaatac gactcactat agggcgaatt
      121 gggtaccggg ccccccctcg aggtcgacgg tatcgataag ctttttcttt tatttaaaat
      181 taaatgtaat atattcacaa ataaaaaaaa acaactaatt tacaactatt taaactaaat
      241 gagccatttt ggtgaaaaga ttttgatata aattataaaa aaaaagtttt aacaaattta
      301 attcacgatc atccaagtta actgaataat tgctgtttgt gaatcggcag ccgttcattt
      361 tcactgagca atgttatttt atttaaacag accctataca cagattttac aacatatgat
      421 ttaaaaaatg tataaacgag atatatgatt aatataggct tcttggcatt ggtcagggac
      481 ccataattcc ctggggccct aggcggctgc taactttgat tattgattaa gtccgctcct
      541 gctagtatgt tcatttgtgc gtccataaaa ggctaattta aacatgttta aaattgtatt
      601 aactacattg accaattatt ttaatttatt aaacatttct acatttccca gaccgcagat
      661 tgaaaccccc aacagtatta aatagatact taatacaggc aagtaaaatc attattaatt
      721 ataattatta ggcctaatta atacatgtat tattatttaa aaaaaatcta atttatatta
      781 aagttaataa a
//
