LOCUS       sequence-148231-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  kdrl promoter for Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Choi J, Dong L, Ahn J, Dao D, Hammerschmidt M, Chen JN
  TITLE     FoxH1 negatively modulates flk1 gene expression and vascular 
            formation in zebrafish.
  JOURNAL   Dev Biol. 2007 Apr 15;304(2):735-44. Epub 2007 Jan 20.
  PUBMED    17306248
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2007-04-15"; volume: "304(2)"; pages: "735-44. Epub 2007 Jan 
            20"
COMMENT     Sequence Label: p5E-kdrl
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..190)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        191..209
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     246..262
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     246..262
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgcggccgcc ctgcagctct agagctcgaa ttctacaggt cactaatacc atctaagtag
       61 ttggttcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      121 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc aacttttttg
      181 tacaaacttg aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
      241 gccgctctag aactagtgga tccactagta acggccgcca gtgtgctgga attcgccctt
      301 gtctgttaaa ataacgtccc gaatgatact ccgtatgtca cttcacgttc ggatgcggct
      361 gcagtaagtt agtccagaac ttaatttttc ttgatgtgtg ataatatgct tatagttcct
      421 gttgatatga tagagatggt tcttcggtga cgtgttaata gttaaacacc tctcagtgac
      481 tgtaaagtat cgcgcaggac tgcgcctgcc ccggtactaa gacgaatgga gaaactccac
      541 tgactccaca actcccgagg gcaggtgcga ccgctgtccg tccttcctaa aaccgaatga
      601 atgaaatttc caaaactaca ctatgtagtg aaggtgtaac agccatttat ctatcttcac
      661 accatcccac tatacctctg acttttctac tgggcttttc cctctcgaag gctttatatg
      721 agtgaagcga ggacggaagg acgcaacgtc accatagtaa ccctgcagcg atactcgtaa
      781 agctacgtgt actgatttaa ctaaatcgat ag
//
