LOCUS       sequence-148235-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Overexpression of Grb10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Kazi JU, Ronnstrand L
  TITLE     FLT3 signals via the adapter protein Grb10 and overexpression of 
            Grb10 leads to aberrant cell proliferation in acute myeloid 
            leukemia.
  JOURNAL   Mol Oncol. 2013 Jun;7(3):402-18. doi: 10.1016/j.molonc.2012.11.003. 
            Epub 2012 Nov 29.
  PUBMED    23246379
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molonc.2012.11.003"; journalName: "Mol Oncol"; date: 
            "2013-06"; volume: "7"; issue: "3"; pages: "402-18"
COMMENT     Sequence Label: pcDNA3-FLAG-Grb10-WT
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cttggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccaccgcca tggactacaa
      121 ggacgatgat gacaagggcg aattcatgaa tgcatccctg gagagcctgt actcggcctg
      181 cagcatgcag tcagacacgg tgcccctcct gcagaatggc cagcatgccc gcagccagcc
      241 tcgggcttca ggccctcctc ggtccatcca gccacaggtg tccccgaggc agagggtgca
      301 gcgctcccag cctgtgcaca tcctcgctgt caggcgcctt caggaggaag accagcagtt
      361 tagaacctca tctctgccgg ccatccccaa tccttttcct gaactctgtg gccctgggag
      421 cccccctgtg ctcacgccgg gttctttacc tccgagccag gccgccgcaa agcaggatgt
      481 taaagtcttt agtgaagatg ggacaagcaa agtggtggag attctagcag acatgacagc
      541 cagagacctg tgccaattgc tggtttacaa aagtcactgt gtggatgaca acagctggac
      601 actagtggag caccacccgc acctaggatt agagaggtgc ttggaagacc atgagctggt
      661 ggtccaggtg gagagtacca tggccagtga gagtaaattt ctattcagga agaattacgc
      721 aaaatacgag ttctttaaaa atcccatgaa tttcttccca gaacagatgg ttacttggtg
      781 ccagcagtca aatggcagtc aaacccagct tttgcagaat tttctgaact ccagtagttg
      841 tcctgaaatt caagggtttt tgcatgtgaa agagctggga aagaaatcat ggaaaaagct
      901 gtatgtgtgt ttgcggagat ctggccttta ttgctccacc aagggaactt caaaggaacc
      961 cagacacctg cagctgctgg ccgacctgga ggacagcaac atcttctccc tgatcgctgg
     1021 caggaa
//
