LOCUS       sequence-148240-        3468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TRAP 4 fused to mEOS3.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3468)
  TITLE     FtsZ-MEEVF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TRAP 4-mEOS
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(278..823)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(358..377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1045..1064
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        1079..1142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1143..1211,1212..2003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1361..1380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(2149..2167,2168..2173,2174..2190,2191..2196,
                     2197..2222)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2149..2167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2174..2192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2232..2243
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2658..3338
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     terminator      3372..3458
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctaggatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
       61 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
      121 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
      181 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
      241 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
      301 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
      361 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
      421 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
      481 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
      601 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
      661 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
      721 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
      781 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
      841 ctgcagagat caattcgcct acgtgaagca ccccgccgac atccccgact acttgaagct
      901 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      961 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1021 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gcttcaaata
     1081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1381 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1681 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gaagatcttc cctatcagtg
     2161 atagagattg acatccctat cagtgataga gatactgagc acatcagcag gacgcactga
     2221 ccgaattcat taaagaggag aaaggtacca tgaagcaggc actgaaagaa aaagagctgg
     2281 ggaacgatgc ctacaagaag aaagactttg acacagcctt gaagcattac gacaaagcca
     2341 aggagctgga ccccactaac atgacttaca ttatcaatca agcagcggta tactttgaaa
     2401 agggcgacta caataagtgc cgggagcttt gtgagaaggc cattgaagtg gggagagaaa
     2461 accgagagga ctatcgatgg attgccattg catatgctcg aattggcaac tcctacttca
     2521 aagaagaaaa gtacaaggat gccatccatt tctataacaa gtctctggca gagcaccgaa
     2581 ccccaaaggt gctaaaaaag tgccaacagg cggagaaaat cctgaaggag caaggcggca
     2641 gcggcagcgg cctgcagatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg
     2701 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
     2761 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
     2821 atatcctgac cactgcattc cattacggca acagggtatt cgccaaatat ccagacaaca
     2881 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
     2941 tcgaagacgg gggcatttgc aacgccagaa acgacataac aatggaaggg gacactttct
     3001 ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga
     3061 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg
     3121 gtgatattga gatggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa
     3181 ctacttacaa agctaaggag aagggtgtca agttaccagg cgcccacttt gtggaccact
     3241 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
     3301 ttgctcattc tggattgcct gacaatgcca gacgatgata aaagcttcat ggtacgcgtg
     3361 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3421 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccct
//
