LOCUS       sequence-148253-         552 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: Ikaros-CRISPR-Nick2/2
            (PX461-EF1a-pSpCas9n(BB)...
FEATURES             Location/Qualifiers
     source          1..552
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(40..59)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(210..230)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(372..391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatatata aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc
       61 attttaaaac ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac
      121 gtattttgta ctaatatctt tgtgtttaca gtcaaattaa ttccaattat ctctctaaca
      181 gccttgtatc gtatatgcaa atatgaagga atcatgggaa ataggccctc acatgtgagc
      241 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      301 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      361 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      421 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      481 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      541 ctgtgtgcac ga
//
