LOCUS       sequence-148254-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid against human Helios.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(164..184)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
       61 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
      121 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
      181 cctcacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tg
//
