LOCUS       sequence-148255-         539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid against human Helios.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
FEATURES             Location/Qualifiers
     source          1..539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        193..404
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
       61 cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg
      121 tgcgccaatt ctgcagacaa atggctctag tggtcttgaa aggagtggga attggctccg
      181 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      241 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      301 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      361 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      421 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      481 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagt
//
