LOCUS       sequence-148259-         485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 NICKASE plasmid against human Helios (2/2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: Helios-CRISPR-Nick 2/2
            (PX461-EF1a-pSpCas9n(BB...
FEATURES             Location/Qualifiers
     source          1..485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(26..274)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(84..103)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(254..274)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(416..435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacttgggtg tgcttgaggt gagccggtgt ttcgtccttt ccacaagata tataaagcca
       61 agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta aaacataatt
      121 ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt tgtactaata
      181 tctttgtgtt tacagtcaaa ttaattccaa ttatctctct aacagccttg tatcgtatat
      241 gcaaatatga aggaatcatg ggaaataggc cctcacatgt gagcaaaagg ccagcaaaag
      301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      481 accgg
//
