LOCUS       sequence-148261-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for GFP with N-terminal 
            BirA-biotinylation signal and TEV celavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMSCV-AVITEV-GFP
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    38..379
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             446..862
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     792..814
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     830..846
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ccctgcccag ggaccaccga cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt
       61 gtctgtctct gtctttgtgc gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta
      121 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
      181 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
      241 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
      301 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
      361 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
      421 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
      481 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
      541 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
      601 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
      661 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
      721 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
      781 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
      841 cctcactcct tctctaggcg ccgga
//
