LOCUS       sequence-148273-         569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid against human NFATc1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
FEATURES             Location/Qualifiers
     source          1..569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(42..61)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(212..232)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(374..393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
       61 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      121 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa
      181 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcacatgtga
      241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      541 ggctgtgtgc acgaaccccc cgttcagcc
//
