LOCUS       sequence-148274-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic 
            domain (CD) linked to IRES-mCherry under the control of TetOp and a 
            minimal CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: TetO-dCas9-mD3A
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga aaataacata
       61 tagacaagcg cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
      121 gtcgagtacc ggtttacaca cacgcaaaat actccttcag cggagcgaag aggtggcgga
      181 tgactggcac gctccatgac cggcccagca gtctctgcct cgccaagcgg ctcatgttgg
      241 agacgtcagt atagtggact gggaaaccaa ataccctttc catttcagtg caccataaga
      301 tgtcctcttt ctcattcatg aagacaggaa aatgctggtc tttgccctgc tttatggagt
      361 ttgacctcgt agtaatggtc ctcactttgc tgaacttggc tatcctgcca tgctccagac
      421 actcctgcag ctccagctta tcattcacag tggatgccaa cggcctgttc ataccgggaa
      481 ggttacccca gaagtagcgg gccctgtgtg cagctgacac ttctttggca tcaatcatca
      541 cagggttgga ctcgagaaat cgcgagatgt ccctcttgtc actaacgccc atggccacca
      601 cattcgcaaa gagccagaag aaggggcgat catctccctc cttgggccgc gcatcatgca
      661 ggaggcggta gaactcaaag aagagccggc cagtgccctc gtagaggccc ttgcgagcag
      721 ggttgacgat ggagaggtca ttgcagggac tgcccccaat caccagatcg aatgggcccc
      781 actcctggat atgcttctgt gtgacgctgc ggacgtcccc gacgtacatg atcttcccct
      841 ggtgccgcac catgcccacc gtgatggagt cctcacacac ctccgaggca atgtagcggt
      901 ccacctgaat gcccaagtcc ttcagcacca ggagccctgt agcgattcca tcaaa
//
