LOCUS       sequence-148275-         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic 
            domain (CD) linked to IRES-mCherry under the control of TetOp and a 
            minimal CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: TetO-dCas9-mD3A
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    127..676
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(286..305)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     515..532
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
       61 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
      121 ccatggtatt atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga
      181 cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat
      241 cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag
      301 agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc
      361 tttgcaggtg tatcttatac acgtggcttt tggccgcagg ggcacctgtc gccaggtggg
      421 gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga
      481 ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga
      541 cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa
      601 gacggcaata tggtggaaaa taacatatag acaagcgcac accggcctta ttccaagcgg
      661 cttcggccag taacgttagg ggggggggtc gagtaccggt ttacacacac gcaaaatact
      721 ccttcagcgg agcgaagagg tggcggatga ctggcacgct ccatgaccgg cccagcagtc
      781 tctgcctcgc caagcggctc atgttggaga cgtcagtata gtggactggg aaaccaaata
      841 ccctttccat ttcagtgcac cataagatgt cctctttctc attcatgaag acaggaaaat
      901 gctggtcttt gccctgcttt atggagtttg acctcgtagt aatggtcctc actttgctga
      961 acttggcta
//
