LOCUS       sequence-148276-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic 
            domain (CD) linked to IRES-mCherry under the control of TetOp and a 
            minimal CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: TetO-dCas9-mD3A
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccccg aattcgccat
       61 acattctaga ggatccgcca ccatggacaa gaagtactcc attgggctcg ctatcggcac
      121 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa
      181 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt
      241 cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac
      301 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tagctaaggt
      361 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca
      421 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc
      481 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt
      541 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga
      601 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa
      661 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag
      721 cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa
      781 gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa
      841 atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga
      901 tgatctcgac aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc
      961 aaagaacctg tcagacgcca ttctgctgag tgatattctg cgagtga
//
