LOCUS       sequence-148278-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) 
            under the control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: pCMV-dCas9-D3A
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt
       61 agctagaccg gtttacacac acgcaaaata ctccttcagc ggagcgaaga ggtggcggat
      121 gactggcacg ctccatgacc ggcccagcag tctctgcctc gccaagcggc tcatgttgga
      181 gacgtcagta tagtggactg ggaaaccaaa taccctttcc atttcagtgc accataagat
      241 gtcctctttc tcattcatga agacaggaaa atgctggtct ttgccctgct ttatggagtt
      301 tgacctcgta gtaatggtcc tcactttgct gaacttggct atcctgccat gctccagaca
      361 ctcctgcagc tccagcttat cattcacagt ggatgccaac ggcctgttca taccgggaag
      421 gttaccccag aagtagcggg ccctgtgtgc agctgacact tctttggcat caatcatcac
      481 agggttggac tcgagaaatc gcgagatgtc cctcttgtca ctaacgccca tggccaccac
      541 attctcaaag agccagaaga aggggcgatc atctccctcc ttgggccgcg catcatgcag
      601 gaggcggtag aactcaaaga agagccggcc agtgccctcg tagaggccct tgcgagcagg
      661 gttgacgatg gagaggtcat tgcagggact gcccccaatc accagatcga atggg
//
