LOCUS       sequence-148281-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the N-terminal fragment of manduca sexta soluble guanylyl 
            cyclase subunits alpha and beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Fritz BG, Roberts SA, Ahmed A, Breci L, Li W, Weichsel A, Brailey 
            JL, Wysocki VH, Tama F, Montfort WR
  TITLE     Molecular model of a soluble guanylyl cyclase fragment determined by
            small-angle X-ray scattering and chemical cross-linking.
  JOURNAL   Biochemistry. 2013 Mar 5;52(9):1568-82. doi: 10.1021/bi301570m. Epub
            2013 Feb 15.
  PUBMED    23363317
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi301570m";
            journalName: "Biochemistry"; date: "2013-03-5- 5"; volume: "52"; 
            issue: "9"; pages: "1568-82"
COMMENT     Sequence Label: pETDuet-msGC-NT13
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agggtaccga cgtcagcgat cgcgtggccg gccgaatctg cagaattcgc cctttcagcg
      121 acggtgacga agttcagtgg ccacgcttat aggcagcact gagtaaagaa gcctgtccta
      181 cttctgtttt tccaattcca gctctcggaa tgtttgttga agtttgtcag tcaaaacttc
      241 cagattctgg gtcagcttat aatcagcttc aaattgctcg gacatgagaa ccaggtctcg
      301 tgtcgcatcg tgaagtggaa tgtcagcaat gcataggccg cggcgagtca gatcgtccaa
      361 attggtcaca cttggataac actggaatac aaccacgtct gtttctggaa tgtacagcat
      421 ttgacctttc aagcgaagag aagcgatttc ctcatgagga tcagtgacac tcatctcttc
      481 aggtttggtt ttcaaaacgt agaccgtgtt aatatgagcc aggacgttag caaacgtcat
      541 ctctaagtgg ggacgcacag tatccaagac atcagtaatc ttacagcctg gtctggtaac
      601 tcttgggagt agacgagaca cagtacgtcc cgcttggacg atgttcagat cacgatcaaa
      661 catcaaatgg aatggaaaca ctcggcagaa tgtcgctgga ctcacttttg gttcaagaga
      721 caacgtttca atctctgcta tctcaggtgc agacacccta ccggtggtgg aagtttcagt
      781 gatcaagaac tggacgtggt cacattcctc tttggtcttt aggatttcca ccttcacttc
      841 ggtgttatgg agtttgctcg ccacagtctt
//
