LOCUS       sequence-148288-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Output plasmid: PPhlF-PAmtR-YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN4036
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        358..407
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     primer_bind     complement(502..523)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(763..782)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
       61 aatactagct ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa
      121 ccgaaaagat tacttcgcgt ttgccacctg acgtctaaga actcggtacc aaaccaatta
      181 ttgaagacgc tgaaaagcgt ctttttttgt ttcggtccgc ttcgacgtac ggtggaatct
      241 gattcgttac caattgacat gatacgaaac gtaccgtatc gttaaggtct tgtccaacca
      301 aatgattcgt taccaattga cagtttctat cgatctatag ataatgctag cctgaagctg
      361 tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa
      421 ttttgtttaa tactagagaa agaggggaaa tactagatgg tgagcaaggg cgaggagctg
      481 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      541 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      601 tgcaccacag gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      661 ctgcaatgct tcgcccgcta ccccgaccac atgaagctgc acgacttctt caagtccgcc
      721 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      781 acccgcgccg aggtgaagtt cgagggcgac acc
//
