LOCUS       sequence-148291-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses the N-terminal fragment of manduca sexta soluble guanylyl 
            cyclase subunits alpha and beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Fritz BG, Roberts SA, Ahmed A, Breci L, Li W, Weichsel A, Brailey 
            JL, Wysocki VH, Tama F, Montfort WR
  TITLE     Molecular model of a soluble guanylyl cyclase fragment determined by
            small-angle X-ray scattering and chemical cross-linking.
  JOURNAL   Biochemistry. 2013 Mar 5;52(9):1568-82. doi: 10.1021/bi301570m. Epub
            2013 Feb 15.
  PUBMED    23363317
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi301570m";
            journalName: "Biochemistry"; date: "2013-03-5- 5"; volume: "52"; 
            issue: "9"; pages: "1568-82"
COMMENT     Sequence Label: pETDuet-msGC-NT21
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             156..173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatgggca gcagccatca ccatcatcac cacagccagg
      181 atccgaccaa agtgacagat ttgaagatcg gcgtggctag cttctgcaaa gcgtttccat
      241 ggcatttcat cactgataaa aggctggaac tcgttcaatt gggagctgga ttcatgcgcc
      301 tcttcggaac ccacttggca acacacgggt cttcgctggg cacgtacttc agactcctgc
      361 gtccaagagg agtgccgctg gactttcgcg agatcctcaa gcgtgtcaac acaccgttca
      421 tgttttgcct aaagatgcct ggttcgacag cacttgccga gggtttggag atcaaaggac
      481 aaatggtgtt ctgtgccgag tcagattctt tgctgtttgt cgggtccccg ttcttggacg
      541 gtctcgaagg tctgactgga aggggactct tcatatccga tatacctctt catgatgcga
      601 cgagagatgt catcttagtc ggagaacagg ctagggctca ggatggtttg cgaagacgaa
      661 tggacaagct gaagaactca atagaagaag cgagcaaagc agtagacaag gaacgagaga
      721 agtaagtcag cctgctgcat ttaatattcc caccccacat cgccaaaagg ctttggctag
      781 gcgagaaaat cgaagctaa
//
