LOCUS       sequence-148293-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Qin H, Hejna M, Liu Y, Percharde M, Wossidlo M, Blouin L, 
            Durruthy-Durruthy J, Wong P, Qi Z, Yu J, Qi LS, Sebastiano V, Song 
            JS, Ramalho-Santos M
  TITLE     YAP Induces Human Naive Pluripotency.
  JOURNAL   Cell Rep. 2016 Mar 15;14(10):2301-12. doi: 
            10.1016/j.celrep.2016.02.036. Epub 2016 Mar 3.
  PUBMED    26947063
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.02.036"; journalName: "Cell Rep"; date: 
            "2016-03-15- 15"; volume: "14"; issue: "10"; pages: "2301-12"
COMMENT     Sequence Label: pGAMA-empty
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(107..231)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             257..298
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             302..355
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      362..371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(513..531)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     781..800
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
       61 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
      121 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      181 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgatctaga
      241 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      301 tgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaccggt
      361 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
      421 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      481 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
      541 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
      601 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
      661 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
      721 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
      781 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtacc
//
