LOCUS       sequence-148296-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Sabourin LA, Rudnicki MA
  TITLE     The molecular regulation of myogenesis.
  JOURNAL   Clin Genet. 2000 Jan . 57(1):16-25.
  PUBMED    10733231
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Genet.
            2000 Jan . 57(1):16-25."
COMMENT     Sequence Label: EMSV-myogenin (puro)
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(46..65)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     439..462
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     complement(556..575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(557..575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(582..599)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(582..598)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(591..613)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
       61 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
      121 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
      181 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
      241 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
      301 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
      361 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
      421 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
      481 aagcgcctcc cctacccggt agaatttcga ggtcgactct agaggatccc cgggtaccga
      541 gctcgaatta attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
      601 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      661 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccc
//
