LOCUS       sequence-148299-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic 
            domain (CD) under the control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: pCMV-dCas9-mD3A
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
       61 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
      121 aagcttgggc tgcaggtcga ctctagagga tccgccacca tggacaagaa gtactccatt
      181 gggctcgcta tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
      241 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
      301 attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
      361 gcacggcgca gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt
      421 aatgagatag ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg
      481 gaggaggata aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg
      541 taccatgaaa agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat
      601 aaggctgact tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac
      661 ttcctcatcg agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa
      721 ctggttcaga cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac
      781 gccaaagcaa tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca
      841 cagctccctg gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg
      901 ctgaccccca actttaaatc taacttcgac ctggccgaag atgccaagct tcaactgagc
      961 aaagacacct acgatgatga tctcgacaat ctgctggccc agatcggcga ccagtacgca
     1021 gacctttttt tggcggcaaa gaacctgtca gacgccattc tgctg
//
