LOCUS       sequence-148301-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic 
            domain (CD) under the control of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: pCMV-dCas9-mD3A
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(77..1009)
                     /codon_start=1
                     /product="catalytic domain of DNA methyltransferase 3A"
                     /label=DNMT3A catalytic domain
                     /translation="PSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATG
                     LLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVI
                     GGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFANVVAMGVS
                     DKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGR
                     IAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSR
                     LARQRLLGRSWSVPVIRHLFAPLKEYFACV"
ORIGIN
        1 aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat atcgaattct
       61 tagctagacc ggtttacaca cacgcaaaat actccttcag cggagcgaag aggtggcgga
      121 tgactggcac gctccatgac cggcccagca gtctctgcct cgccaagcgg ctcatgttgg
      181 agacgtcagt atagtggact gggaaaccaa ataccctttc catttcagtg caccataaga
      241 tgtcctcttt ctcattcatg aagacaggaa aatgctggtc tttgccctgc tttatggagt
      301 ttgacctcgt agtaatggtc ctcactttgc tgaacttggc tatcctgcca tgctccagac
      361 actcctgcag ctccagctta tcattcacag tggatgccaa cggcctgttc ataccgggaa
      421 ggttacccca gaagtagcgg gccctgtgtg cagctgacac ttctttggca tcaatcatca
      481 cagggttgga ctcgagaaat cgcgagatgt ccctcttgtc actaacgccc atggccacca
      541 cattcgcaaa gagccagaag aaggggcgat catctccctc cttgggccgc gcatcatgca
      601 ggaggcggta gaactcaaag aagagccggc cagtgccctc gtagaggccc ttgcgagcag
      661 ggttgacgat ggagaggtca ttgcagggac tgcccccaat caccagatcg aatgggcccc
      721 actcctggat atgcttctgt gtgacgctgc ggacgtcccc gacgtacatg atcttcccct
      781 ggtgccgcac catgcccacc gtgatggagt cctcacacac ctccgaggca atgtagcggt
      841 ccacctgaat gcccaagtcc ttcagcacca ggagccctgt agcgattcca tcaaagagag
      901 acagcacccg gatgggcttc ctcttctcag ctgggacagg tgggtaaacc tttggagggt
      961 caaattcctg gtcgtggtta ttagcgaaga acatctggag ccgggagggc cagtcctctc
     1021 ggcttccctt gtcgtcatcg t
//
