LOCUS       sequence-148304-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP expression plasmid (also called p111) for the creation of a 
            heteroduplex DNA repair assay plasmid with p189. *Please see notes 
            in comments section..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Zhou B, Huang C, Yang J, Lu J, Dong Q, Sun LZ
  TITLE     Preparation of heteroduplex enhanced green fluorescent protein 
            plasmid for in vivo mismatch repair activity assay.
  JOURNAL   Anal Biochem. 2009 May 1;388(1):167-9. doi: 
            10.1016/j.ab.2009.02.020. Epub 2009 Feb 25.
  PUBMED    19248754
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2009.02.020"; journalName: "Anal Biochem"; date: 
            "2009-05-1- 1"; volume: "388"; issue: "1"; pages: "167-9"
COMMENT     Sequence Label: pGEM5Z(+)-EGFP
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(11..30)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    40..121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     65..84
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(289..310)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     638..657
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
       61 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      121 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      181 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc
      241 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      301 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      361 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      421 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      481 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      541 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      601 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      661 gaa
//
