LOCUS       sequence-148308-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses human optimized S. pyogenes dCas9 and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: pLV hUbC-dCas9-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..70)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(77..97)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccttgctcac ggtaccaggg ccgggattct cctccacgtc accgcatgtt agaagacttc
       61 ctctgccctc gctagccacc ttcctcttct tcttggggtc agccctgctg tctccaccga
      121 gctgagagag gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg
      181 cgtccaggac ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact
      241 tgaaggctgc aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct
      301 gctccctgat gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag
      361 cgtcggcgag gatcactctt ttggagaatt cgcttatttg ctcgatgatc tcatcaaggt
      421 agtgtttgtg ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga
      481 gcttttcata gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct
      541 cgttaccttt ctgcagctcg cccgcactag cgagcattcg tttccggccg ttttcaagct
      601 caaagagaga gtacttggga agcttaatga tgaggtcttt tttgacctct ttatatcctt
      661 tcgcctcgag aaagtcgatg gggtttt
//
