LOCUS       sequence-148374-         433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: NFATc2-CRISPR-Nick 1/2
            (PX461-EF1a-pSpCas9n(BB...
FEATURES             Location/Qualifiers
     source          1..433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..51)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(202..222)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(364..383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa
       61 acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg
      121 tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta
      181 tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcacatgtga gcaaaaggcc
      241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      421 tgccgcttac cgg
//
